Part:BBa_K4351018:Design

Glucose binding RNA aptamer 1 + RNA Mango

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 98
  

Design Notes

Need T7 promoter for in vitro use

Source

Yang KA, Barbu M, Halim M, Pallavi P, Kim B, Kolpashchikov DM, Pecic S, Taylor S, Worgall TS, Stojanovic MN. Recognition and sensing of low-epitope targets via ternary complexes with oligonucleotides and synthetic receptors. Nat Chem. 2014 Nov;6(11):1003-8. doi: 10.1038/nchem.2058. Epub 2014 Sep 28. PMID: 25343606; PMCID: PMC4339820.

Dolgosheina EV, Jeng SCY, Panchapakesan SSS, Cojocaru R, Chen PSK, Wilson PD, Hawkins N, Wiggins PA, and Unrau PJ. ACS Chemical Biology 2014 9 (10), 2412-2420 DOI: 10.1021/cb500499x